| | 8. | Cadherin-Based Adhesion Is a Potential Target for Niche Manipulation to Protect Hematopoietic Stem Cells in Adult Bone Marrow
Pages 194-198
Kentaro Hosokawa, Fumio Arai, Hiroki Yoshihara, Hiroko Iwasaki, Mark Hembree, Tong Yin, Yuka Nakamura, Yumiko Gomei, Keiyo Takubo, Haruko Shiama, Sahoko Matsuoka, Linheng Li, Toshio Suda
| | | | 12. | Conserved and Divergent Roles of FGF Signaling in Mouse Epiblast Stem Cells and Human Embryonic Stem Cells
Pages 215-226
Boris Greber, Guangming Wu, Christof Bernemann, Jin Young Joo, Dong Wook Han, Kinarm Ko, Natalia Tapia, Davood Sabour, Jared Sterneckert, Paul Tesar, Hans R. Schöler
Graphical Abstract
► Nanog is a direct target of Activin and SMAD2/3 but not FGF-ERK in EpiSCs ► FGF signaling inhibits neuroectodermal commitment of EpiSCs and hESCs ► FGF inhibition relieves Klf2 repression and reverts EpiSCs to an ESC-like state ► mESCs transition to an EpiSC-like state with LIF inhibition and FGF activation
| | | | 13. | G-CSF Promotes the Proliferation of Developing Cardiomyocytes In Vivo and in Derivation from ESCs and iPSCs
Pages 227-237
Kenichiro Shimoji, Shinsuke Yuasa, Takeshi Onizuka, Fumiyuki Hattori, Tomofumi Tanaka, Mie Hara, Yohei Ohno, Hao Chen, Toru Egasgira, Tomohisa Seki, Kojiro Yae, Uichi Koshimizu, Satoshi Ogawa, Keiichi Fukuda
Graphical Abstract► G-CSF is strongly expressed in developing cardiomyocytes ► G-CSF promotes cardiomyocyte proliferation in vivo ► In vitro, G-CSF boosts yields of ESC- and iPSC-derived cardiomyocytes ► G-CSF is acting to promote proliferation through JAK-STAT signaling
| | | | 14. | Embryonic MGE Precursor Cells Grafted into Adult Rat Striatum Integrate and Ameliorate Motor Symptoms in 6-OHDA-Lesioned Rats
Pages 238-250
Verónica Martínez-Cerdeño, Stephen C. Noctor, Ana Espinosa, Jeanelle Ariza, Philip Parker, Samantha Orasji, Marcel M. Daadi, Krystof Bankiewicz, Arturo Alvarez-Buylla, Arnold R. Kriegstein
Graphical Abstract► Embryonic MGE cells were transplanted into the adult striatum of 6-OHDA-treated rats ► MGE cells transplanted into the adult striatum differentiate into GABAergic neurons ► MGE cells integrate and modify striatal-dependent motor behavior ► MGE cells ameliorate motor symptoms in the rat 6-OHDA model of Parkinson's disease
| | | | 15. | Endothelial Cells Are Essential for the Self-Renewal and Repopulation of Notch-Dependent Hematopoietic Stem Cells
Pages 251-264
Jason M. Butler, Daniel J. Nolan, Eva L. Vertes, Barbara Varnum-Finney, Hideki Kobayashi, Andrea T. Hooper, Marco Seandel, Koji Shido, Ian A. White, Mariko Kobayashi, Larry Witte, Chad May, Carrie Shawber, Yuki Kimura, Jan Kitajewski, Zev Rosenwaks, Irwin D. Bernstein, Shahin Rafii
Graphical Abstract
► Coculture with endothelial cells promotes HSC expansion and self-renewal ► Endothelial cell effect on HSCs mediated by Notch signaling ► In vivo, sinusoidal endothelial cells (SECs) express Notch ligands ► Blocking vessel remodeling impairs hematopoietic recovery after irradiation
| | | | 16. | Distinct Hematopoietic Stem Cell Subtypes Are Differentially Regulated by TGF-β1
Pages 265-278
Grant A. Challen, Nathan C. Boles, Stuart M. Chambers, Margaret A. Goodell
Graphical Abstract► Myeloid HSC (My-HSC) and lymphoid HSC (Ly-HSC) bias in subfractions of purified HSCs ► Lineage bias persists through serial transplantation ► CD150 further distinguishes HSC subpopulations ► Ly-HSCs and My-HSCs show differential responses to TGF-β1
| | | | 17. | Poised Lineage Specification in Multipotential Hematopoietic Stem and Progenitor Cells by the Polycomb Protein Bmi1
Pages 279-286
Hideyuki Oguro, Jin Yuan, Hitoshi Ichikawa, Tomokatsu Ikawa, Satoshi Yamazaki, Hiroshi Kawamoto, Hiromitsu Nakauchi, Atsushi Iwama
Graphical Abstract► Loss of Bmi1 causes premature activation of lineage-specific genes in HSCs ► Bmi1 reinforces bivalent chromatin domains at key developmental regulator gene loci ► Loss of Bmi1 enhances B cell lineage differentiation at the expense of T cell lineage ► Bmi1 inhibits HSC lineage specification to maintain the multipotent state
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