| | 10. | The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility
Pages 631-642
Lillian K. Fritz-Laylin, Simon E. Prochnik, Michael L. Ginger, Joel B. Dacks, Meredith L. Carpenter, Mark C. Field, Alan Kuo, Alex Paredez, Jarrod Chapman, Jonathan Pham, Shengqiang Shu, Rochak Neupane, Michael Cipriano, Joel Mancuso, Hank Tu, Asaf Salamov, Erika Lindquist, Harris Shapiro, Susan Lucas, Igor V. Grigoriev, W. Zacheus Cande, Chandler Fulton, Daniel S. Rokhsar, Scott C. Dawson
Graphical Abstract
► Naegleria's genome sequence suggests a versatile and complex ancestral eukaryote ► The genome encodes both aerobic and anaerobic hydrogen-producing metabolism ► We predict novel evolutionarily conserved genes associated with amoeboid motility ► Genomic comparisons identify 4133 protein families likely ancestral to all eukaryotes
| | | | 12. | miR-328 Functions as an RNA Decoy to Modulate hnRNP E2 Regulation of mRNA Translation in Leukemic Blasts
Pages 652-665
Anna M. Eiring, Jason G. Harb, Paolo Neviani, Christopher Garton, Joshua J. Oaks, Riccardo Spizzo, Shujun Liu, Sebastian Schwind, Ramasamy Santhanam, Christopher J. Hickey, Heiko Becker, Jason C. Chandler, Raul Andino, Jorge Cortes, Peter Hokland, Claudia S. Huettner, Ravi Bhatia, Denis C. Roy, Stephen A. Liebhaber, Michael A. Caligiuri, Guido Marcucci, Ramiro Garzon, Carlo M. Croce, George A. Calin, Danilo Perrotti
Graphical Abstract
► Expression of miR-328 is reduced in CD34+ CML blast crisis (CML-BC) progenitors ► miR-328 directly binds hnRNP E2 and rescues CEBPA mRNA translation ► miR-328 restores C/EBPα-dependent maturation of myeloid CML-BC progenitors ► miR-328 pairs with PIM1 3′UTR and impairs survival of CML-BC progenitors
| | | | 13. | Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity
Pages 666-677
Elizabeth H. Bayne, Sharon A. White, Alexander Kagansky, Dominika A. Bijos, Luis Sanchez-Pulido, Kwang-Lae Hoe, Dong-Uk Kim, Han-Oh Park, Chris P. Ponting, Juri Rappsilber, Robin C. Allshire
Graphical Abstract
► Stc1 is required for RNAi-dependent heterochromatin formation in fission yeast ► Stc1 associates with both RNAi and chromatin modification subunits ► Stc1 localizes to centromeric heterochromatin, dependent on RNAi ► Tethering Stc1 to DNA induces heterochromatin formation independently of RNAi
| | | | 14. | Distinct Factors Control Histone Variant H3.3 Localization at Specific Genomic Regions
Pages 678-691
Aaron D. Goldberg, Laura A. Banaszynski, Kyung-Min Noh, Peter W. Lewis, Simon J. Elsaesser, Sonja Stadler, Scott Dewell, Martin Law, Xingyi Guo, Xuan Li, Duancheng Wen, Ariane Chapgier, Russell C. DeKelver, Jeffrey C. Miller, Ya-Li Lee, Elizabeth A. Boydston, Michael C. Holmes, Philip D. Gregory, John M. Greally, Shahin Rafii, Chingwen Yang, Peter J. Scambler, David Garrick, Richard J. Gibbons, Douglas R. Higgs, Ileana M. Cristea, Fyodor D. Urnov, Deyou Zheng, C. David Allis
Graphical Abstract
► H3.3 localization at specific genes and regulatory regions is cell-type specific ► Hira controls H3.3 localization to genes and some regulatory regions ► H3.3 localization to many regulatory elements and telomeres is Hira independent ► Atrx is required for H3.3 telomeric localization, and for telomeric RNA repression
| | | | 15. | The Tetrahymena Argonaute-Binding Protein Giw1p Directs a Mature Argonaute-siRNA Complex to the Nucleus
Pages 692-703
Tomoko Noto, Henriette M. Kurth, Kensuke Kataoka, Lucia Aronica, Leroi V. DeSouza, K.W. Michael Siu, Ronald E. Pearlman, Martin A. Gorovsky, Kazufumi Mochizuki
Graphical Abstract
► siRNA-directed DNA elimination in Tetrahymena requires Twi1p, an Argonaute protein ► We have uncovered Giw1p as a factor required for macronuclear localization of Twi1p ► Twi1p's slicer activity removes siRNA passenger strand, allowing Giw1p to bind Twi1p ► Twi1p slicer activity and Giw1p are required for the programmed DNA elimination
| | | | 16. | The Angelman Syndrome Protein Ube3A Regulates Synapse Development by Ubiquitinating Arc
Pages 704-716
Paul L. Greer, Rikinari Hanayama, Brenda L. Bloodgood, Alan R. Mardinly, David M. Lipton, Steven W. Flavell, Tae-Kyung Kim, Eric C. Griffith, Zachary Waldon, Rene Maehr, Hidde L. Ploegh, Shoaib Chowdhury, Paul F. Worley, Judith Steen, Michael E. Greenberg
Graphical Abstract
► Ube3A expression is induced by experience-driven synaptic activity ► Ube3A ubiquitinates and degrades Arc promoting AMPAR expression at synapses ► Ube3A KOs have increased Arc levels and thus reduced AMPAR expression at synapses
| | | | 17. | The lta4h Locus Modulates Susceptibility to Mycobacterial Infection in Zebrafish and Humans
Pages 717-730
David M. Tobin, Jay C. Vary Jr., John P. Ray, Gregory S. Walsh, Sarah J. Dunstan, Nguyen D. Bang, Deanna A. Hagge, Saraswoti Khadge, Mary-Claire King, Thomas R. Hawn, Cecilia B. Moens, Lalita Ramakrishnan
Graphical Abstract
► Mutations in zebrafish lta4h result in hypersusceptibility to mycobacterial infection ► LTA4H activity orchestrates the balance of pro- and anti-inflammatory eicosanoids ► Excess Lipoxin A4 production inhibits TNF induction during infection ► In humans, heterozygosity at the LTA4H locus protects from tuberculosis and leprosy
| | | | 18. | Genome-wide Analysis of the Host Intracellular Network that Regulates Survival of Mycobacterium tuberculosis
Pages 731-743
Dhiraj Kumar, Lekha Nath, Md. Azhar Kamal, Ankur Varshney, Avinash Jain, Sarman Singh, Kanury V.S. Rao
Graphical Abstract
► A genome-wide siRNA screen identified host factors regulating M. tuberculosis infection ► These factors define various functional modules of the host human macrophage ► A core set of regulatory factors were identified with a panel of Mtb field isolates ► These core host cell factors predominantly function in the regulation of autophagy
| | | | 19. | An Atlas of Combinatorial Transcriptional Regulation in Mouse and Man
Pages 744-752
Timothy Ravasi, Harukazu Suzuki, Carlo Vittorio Cannistraci, Shintaro Katayama, Vladimir B. Bajic, Kai Tan, Altuna Akalin, Sebastian Schmeier, Mutsumi Kanamori-Katayama, Nicolas Bertin, Piero Carninci, Carsten O. Daub, Alistair R.R. Forrest, Julian Gough, Sean Grimmond, Jung-Hoon Han, Takehiro Hashimoto, Winston Hide, Oliver Hofmann, Hideya Kawaji, Atsutaka Kubosaki, Timo Lassmann, Erik van Nimwegen, Chihiro Ogawa, Rohan D. Teasdale, Jesper Tegnér, Boris Lenhard, Sarah A. Teichmann, Takahiro Arakawa, Noriko Ninomiya, Kayoko Murakami, Michihira Tagami, Shiro Fukuda, Kengo Imamura, Chikatoshi Kai, Ryoko Ishihara, Yayoi Kitazume, Jun Kawai, David A. Hume, Trey Ideker, Yoshihide Hayashizaki
Graphical Abstract
► An atlas of human and mouse transcription factor interactions ► Quantification of transcription factor expression across human and mouse tissues ► A network of 15 transcription factors predicts tissue type specification ► SMAD3/FLI1 forms a repressor complex that controls monocyte differentiation
| | | | 20. | Reduced IGF-1 Signaling Delays Age-Associated Proteotoxicity in Mice
Page 753
Ehud Cohen, Johan F. Paulsson, Pablo Blinder, Tal Burstyn-Cohen, Deguo Du, Gabriela Estepa, Anthony Adame, Hang M. Pham, Martin Holzenberger, Jeffery W. Kelly, Eliezer Masliah, Andrew Dillin
| | |
