| | 8. | Acid Extrusion from Human Spermatozoa Is Mediated by Flagellar Voltage-Gated Proton Channel
Pages 327-337
Polina V. Lishko, Inna L. Botchkina, Andriy Fedorenko, Yuriy Kirichok
Graphical Abstract
► Whole-cell patch-clamp recordings of human spermatozoa reveal high proton conductance ► This conductance is due to proton channel Hv1 located in the sperm flagellum ► Voltage, an alkaline environment, and anandamide activate Hv1 while zinc inhibits it ► Hv1 activation induces intracellular alkalinization, known to activate sperm motility
| | | | 9. | Double-Stranded RNA-Dependent Protein Kinase Links Pathogen Sensing with Stress and Metabolic Homeostasis
Pages 338-348
Takahisa Nakamura, Masato Furuhashi, Ping Li, Haiming Cao, Gurol Tuncman, Nahum Sonenberg, Cem Z. Gorgun, Gökhan S. Hotamisligil
Graphical Abstract
► Double-stranded RNA-dependent protein kinase (PKR) activity is elevated in obese mice ► Excess nutrients and endoplasmic reticulum stress also activate PKR ► JNK activation and inhibition of insulin signaling due to metabolic stress require PKR ► Lack of PKR prevents insulin resistance and metabolic dysfunction in obesity
| | | | 10. | Treslin Collaborates with TopBP1 in Triggering the Initiation of DNA Replication
Pages 349-359
Akiko Kumagai, Anna Shevchenko, Andrej Shevchenko, William G. Dunphy
Graphical Abstract
► Treslin is a newly identified factor for initiation of vertebrate DNA replication ► Treslin interacts with TopBP1, a protein required for initiation of DNA replication ► Association of Treslin and TopBP1 requires cyclin-dependent kinase activity ► Treslin and TopBP1 collaborate in loading the initiator protein Cdc45 onto DNA
| | | | 11. | Nucleoporins Directly Stimulate Expression of Developmental and Cell-Cycle Genes Inside the Nucleoplasm
Pages 360-371
Bernike Kalverda, Helen Pickersgill, Victor V. Shloma, Maarten Fornerod
Graphical Abstract
► Genes interact with nuclear pore proteins (Nups) at the pores and in the nucleoplasm ► Genes interacting with nuclear pore complexes are not preferentially active ► Nups in the nucleoplasm predominantly associate with active genes in Drosophila ► Nucleoplasmic Nups stimulate expression of developmental and cell-cycle genes
| | | | 12. | Chromatin-Bound Nuclear Pore Components Regulate Gene Expression in Higher Eukaryotes
Pages 372-383
Maya Capelson, Yun Liang, Roberta Schulte, William Mair, Ulrich Wagner, Martin W. Hetzer
Graphical Abstract
► Nucleoporins (Nups) bind to active and silent loci in the Drosophila genome ► Genes can interact with Nups not associated with the nuclear pore complex (NPC) ► Sec13, Nup98, and FG-repeat Nups are recruited to sites of active transcription ► NPC components are required for the expression of developmentally regulated genes
| | | | 13. | USP10 Regulates p53 Localization and Stability by Deubiquitinating p53
Pages 384-396
Jian Yuan, Kuntian Luo, Lizhi Zhang, John C. Cheville, Zhenkun Lou
Graphical Abstract
► USP10 is a deubiquitinase specific for p53 and counteracts Mdm2 ► Upon DNA damage, USP10 translocates to the nucleus and stabilizes p53 ► USP10 translocation requires ATM-mediated phosphorylation ► USP10 acts as a tumor suppressor in cells with wild-type p53
| | | | 14. | RIG-I Detects Viral Genomic RNA during Negative-Strand RNA Virus Infection
Pages 397-408
Jan Rehwinkel, Choon Ping Tan, Delphine Goubau, Oliver Schulz, Andreas Pichlmair, Katja Bier, Nicole Robb, Frank Vreede, Wendy Barclay, Ervin Fodor, Caetano Reis e Sousa
Graphical Abstract
► RIG-I is a sensor for virus infection that triggers antiviral responses ► RIG-I binds replicated viral genomes in influenza- and Sendai virus-infected cells ► These single-stranded viral genomes are the natural RIG-I agonists ► 5′-triphosphate groups on viral genomes are critical for RIG-I activation
| | | | 16. | Aplysia CPEB Can Form Prion-like Multimers in Sensory Neurons that Contribute to Long-Term Facilitation
Pages 421-435
Kausik Si, Yun-Beom Choi, Erica White-Grindley, Amitabha Majumdar, Eric R. Kandel
Graphical Abstract
► The Aplysia translational regulator ApCPEB has prion-like properties in neurons ► Exogenously expressed ApCPEB forms self-sustaining amyloidogenic multimers ► Serotonin enhances formation of exogenous ApCPEB multimers ► Inhibition of ApCPEB multimers blocks persistence of long-term facilitation
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