: J Immunol. 2007 Aug 1;179(3):1855-
Intrapulmonary delivery of bone marrow-derived mesenchymal stem cells
improves survival and attenuates endotoxin-induced acute lung injury
in mice.Gupta N, Su X, Popov B, Lee JW, Serikov V, Matthay MA.
Cardiovascular Research Institute, University of California, San
Francisco, CA 94143.
Recent in vivo and in vitro work suggests that mesenchymal stem cells
(MSC) have anti-inflammatory properties. In this study, we tested the
effect of administering MSC directly into the airspaces of the lung 4
h after the intrapulmonary administration of Escherichia coli
endotoxin (5 mg/kg). MSC increased survival compared with PBS-treated
control mice at 48 h (80 vs 42%; p < 0.01). There was also a
significant decrease in excess lung water, a measure of pulmonary
edema (145 +/- 50 vs 87 +/- 20 mul; p < 0.01), and bronchoalveolar
lavage protein, a measure of endothelial and alveolar epithelial
permeability (3.1 +/- 0.4 vs 2.2 +/- 0.8 mg/ml; p < 0.01), in the MSC-
treated mice. These protective effects were not replicated by the use
of further controls including fibroblasts and apoptotic MSC. The
beneficial effect of MSC was independent of the ability of the cells
to engraft in the lung and was not related to clearance of the
endotoxin by the MSC. MSC administration mediated a down-regulation
of proinflammatory responses to endotoxin (reducing TNF-alpha and MIP-
2 in the bronchoalveolar lavage and plasma) while increasing the anti-
inflammatory cytokine IL-10. In vitro coculture studies of MSC with
alveolar macrophages provided evidence that the anti-inflammatory
effect was paracrine and was not cell contact dependent. In
conclusion, treatment with intrapulmonary MSC markedly decreases the
severity of endotoxin-induced acute lung injury and improves survival
in mice.
PMID: 17641052 [PubMed - in process]
http://www.ncbi.
Db=pubmed&Cmd=
=EntrezSystem2.
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StemCells subscribers may also be interested in these sites:
Children's Neurobiological Solutions
http://www.CNSfoundation.org/
Cord Blood Registry
http://www.CordBlood.com/at.cgi?a=150123
The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
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