Postnatal stem/progenitor cells derived from the dental pulp of adult
chimpanzee
Chimpanzee dental pulp stem/stromal cells (ChDPSCs) are very similar
to human bone marrow derived mesenchymal stem/stromal cells (hBMSCs)
as demonstrated by the expression pattern of cell surface markers and
their multipotent differentiation capability.
Results: ChDPSCs were isolated from an incisor and a canine of a
forty-seven year old female chimpanzee.
A homogenous population of ChDPSCs was established in early culture
at a high proliferation rate and verified by the expression pattern
of thirteen cell surface markers. The ChDPSCs are multipotent and
were capable of differentiating into osteogenic, adipogenic and
chondrogenic lineages under appropriate in vitro culture conditions.
ChDPSCs also express stem cell (Sox-2, Nanog, Rex-1, Oct-4) and
osteogenic (Osteonectin, osteocalcin, osteopontin) markers, which is
comparable to reported results of rhesus monkey BMSCs (rBMSCs),
hBMSCs and hDPSCs. Although ChDPSCs vigorously proliferated during
the initial phase and gradually decreased in subsequent passages, the
telomere length indicated that telomerase activity was not
significantly reduced.
Conclusions: These results demonstrate that ChDPSCs can be
efficiently isolated from post-mortem teeth of adult chimpanzees and
are multipotent. Due to the almost identical genome composition of
humans and chimpanzees, there is an emergent need for defining the
new role of chimpanzee modeling in comparative medicine.
Teeth are easy to recover at necropsy and easy to preserve prior to
the retrieval of dental pulp for stem/stromal cells isolation.
Therefore, the establishment of ChDPSCs would preserve and maximize
the applications of such a unique and invaluable animal model, and
could advance the understanding of cellular functions and
differentiation control of adult stem cells in higher primates.
http://7thspace.
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Author: Pei-Hsun Cheng, Brooke Snyder, Dimitri Fillos, Chris C
Ibegbu, Anderson Hsien-Cheng Huang and Anthony W.S. Chan
Credits/Source: BMC Cell Biology 2008, 9:20
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StemCells subscribers may also be interested in these sites:
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http://www.CNSfoundation.org/
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http://www.CordBlood.com/at.cgi?a=150123
The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
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