Study: Cryopreservation of transplantable cultured limbal stem cells
possible
Limbal stem cells expanded on amniotic membrane can be cryopreserved
for potential transplantation to treat patients with limbal stem cell
deficiency, according to a study published in the April issue of
Cornea.
Huey-Chuan Cheng, MD, and colleagues at National Cheng Kung
University in Taiwan cultured pieces of limbus on amniotic membrane
for 3 weeks to grow limbal stem cells. They then tested different
cryoprotectant formulations for preserving the resulting cell sheets
in liquid nitrogen.
"Expanded cell sheets usually exhibited [three to six] stratified
layers after 3-week culture on [amniotic membrane] and expressed
specific markers of ABCG2 and vimentin for limbal stem cells," the
authors said.
The researchers found that the optimal formula for preserving the
expanded cell sheets included 60% Dulbecco modified Eagle medium, 30%
fetal bovine serum and 10% dimethyl sulfoxide.
The stem cells maintained their cellular characteristics after 8
weeks of cryopreservation and, when thawed, showed an average 53.8%
cell viability, according to the study.
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StemCells subscribers may also be interested in these sites:
Children's Neurobiological Solutions
http://www.CNSfoundation.org/
Cord Blood Registry
http://www.CordBlood.com/at.cgi?a=150123
The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
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