May 2008 Volume 5 Number 5, pp 371 - 455
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EDITORIAL
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Talking points pp371 - 372
Presenting at a conference is a unique opportunity to broadly
communicate your work. Here are ten suggestions to make
the most of it.
doi:10.1038/nmeth0508-371
http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsC0E1
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CORRESPONDENCE
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Two-photon dual-color imaging using fluorescent proteins
pp373 - 374
Hiroyuki Kawano et al.
doi:10.1038/nmeth0508-373
http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsD0E2
Unexpected failure rates for modular assembly of engineered zinc
fingers pp374 - 375
Cherie L Ramirez et al.
doi:10.1038/nmeth0508-374
http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsE0E3
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RESEARCH HIGHLIGHTS
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Two pictures are better than one p377
Daniel Evanko
doi:10.1038/nmeth0508-377
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsF0E4
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsG0E5
Protein structure determination... blindfolded pp378 - 379
Allison Doerr
doi:10.1038/nmeth0508-378a
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsH0E6
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsI0E7
Knockouts with a touch of a zinc finger pp378 - 379
Irene Kaganman
doi:10.1038/nmeth0508-378b
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsJ0E8
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsK0EA
News in brief p379
doi:10.1038/nmeth0508-379
http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsL0EB
Follow the ubiquitin p380
Katherine Stevens
doi:10.1038/nmeth0508-380
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsM0EC
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsN0ED
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NEWS AND VIEWS
----------------------
BAC to the future: functional genomics in mammals pp383 - 384
A high-throughput pipeline to engineer bacterial artificial
chromosomes (BACs) expressing tagged genes of higher eukaryotes
allows large-scale protein localization and interaction studies.
Assen Roguev and Nevan J Krogan
doi:10.1038/nmeth0508-383
http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsO0EE
Metastasis: two assays explore the two roads traveled pp384 - 385
A spheroid assay that recapitulates angiogenesis in vivo and a ring
assay to measure lymphangiogenesis in vitro expand the toolbox of
techniques to investigate these processes during development and
tumor progression.
Diane Renee Bielenberg
doi:10.1038/nmeth0508-384
http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsP0EF
Super-resolution light microscopy goes live pp385 - 387
Microscopic resolution far beyond the diffraction limit is possible
by localizing single molecules individually. This approach has now
been demonstrated on living cells.
Mats G L Gustafsson
doi:10.1038/nmeth0508-385
http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsQ0EG
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BRIEF COMMUNICATIONS
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Improved genetic manipulation of human embryonic stem
cells pp389 - 392
Stefan R Braam et al.
doi:10.1038/nmeth.1200
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsR0EH
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsS0EI
Suspended-drop electroporation for high-throughput delivery of
biomolecules into cells pp393 - 395
Emmanuel G Guignet and Tobias Meyer
doi:10.1038/nmeth.1201
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsT0EJ
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsU0EK
Monovalent, reduced-size quantum dots for imaging receptors on living
cells pp397 - 399
Mark Howarth et al.
doi:10.1038/nmeth.1206
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsV0EL
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsW0EM
Fluorescent protein FRET pairs for ratiometric imaging of dual
biosensors pp401 - 403
Hui-wang Ai, Kristin L Hazelwood, Michael W Davidson and Robert
E Campbell
doi:10.1038/nmeth.1207
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsX0EN
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsY0EO
Straightforward ladder sequencing of peptides using a Lys-N
metalloendopeptidase pp405 - 407
Nadia Taouatas, Madalina M Drugan, Albert J R Heck and Shabaz
Mohammed
doi:10.1038/nmeth.1204
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80BtsZ0EP
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsa0EW
----------------------
ARTICLES
----------------------
BAC TransgeneOmics: a high-throughput method for exploration of
protein function in mammals pp409 - 415
Ina Poser et al.
doi:10.1038/nmeth.1199
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsb0EX
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsc0EY
Live-cell photoactivated localization microscopy of nanoscale adhesion
dynamics pp417 - 423
Hari Shroff, Catherine G Galbraith, James A Galbraith and Eric Betzig
doi:10.1038/nmeth.1202
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsd0EZ
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btse0Ea
A metric for odorant comparison pp425 - 429
Rafi Haddad et al.
doi:10.1038/nmeth.1197
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsf0Eb
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsg0Ec
Modeling lymphangiogenesis in a three-dimensional culture
system pp431 - 437
Francoise Bruyere et al.
doi:10.1038/nmeth.1205
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsh0Ed
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsi0Ee
Spheroid-based engineering of a human vasculature in
mice pp439 - 445
Abdullah Alajati et al.
doi:10.1038/nmeth.1198
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsj0Ef
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsk0Eg
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TECHNOLOGY FEATURE
----------------------
SNP genotyping: six technologies that keyed a
revolution pp447 - 453
With abundant sequencing data, falling prices and mature genotyping
platforms, researchers have more options than ever to explore the
connections between genes and phenotype.
Jeffrey Perkel
doi:10.1038/nmeth0508-447
http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsl0Eh
----------------------
ADDENDUM
----------------------
Addendum: Highly inclined thin illumination enables clear
single-molecule imaging in cells p455
Makio Tokunaga, Naoko Imamoto and Kumiko Sakata-Sogawa
doi:10.1038/nmeth0508-455
http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsm0Ei
----------------------
APPLICATION NOTES
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3K Long-Tag Paired End sequencing with the Genome Sequencer FLX System
Thomas Jarvie and Timothy Harkins
doi:10.1038/nmeth.f.212
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsn0Ej
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btso0Ek
High-content analysis with AxioVision ASSAYbuildertrade
Horst Wolff
doi:10.1038/nmeth.f.213
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsp0El
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ekRM0Xztnp0Hi80Btsq0Em
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