Dear Colleague:
The FAES Bio-Trac program will be conducting a hands-on "TRAC 23: Flow Cytometry" workshop at the NIH campus on Tuesday, November 13 – Friday, November 16, 2007. This workshop will include lecture and hands-on laboratory exercises. Each participant will receive a comprehensive binder containing all material presented in the workshop along with laboratory protocols and reference material. Space is limited, we will only be taking 30 participants.
Registration information can be obtained from Bea Sonnenberg at FAES (301-496-2316). Course schedule and content information can be obtained from Mark Nardone at 301-496-8290, nardonem@mail.nih.gov or from the Bio-Trac website at www.biotrac.com. If you desire a copy of the current Bio-Trac flyer or a course schedule, please request by email. Please forward to others who might be interested
Regards,
Mark Nardone
FAES/NIH
Director
Bio-Trac Programs
301-496-829
SPONSORED BY
FAES (Foundation for The Advanced Education in the Sciences, Inc.)
at the National Institutes of Health
Bio-Trac 23 – Flow Cytometry: Principles and Methods
Lecture and "Hands-on" Laboratory Training Course
Tuesday, November 13 – Friday, November 16, 2007 (28 hours of lecture and lab training)
4 Day Workshop Fee: $795
This 4-day lecture/laboratory course will cover various applications for flow cytometry in basic research. Selected topics of the lectures will provide a broad background about the functions of a flow cytometer; choice of fluorochromes; multi-color data presentation; technical protocols for flow cytometric procedures and trouble shooting during data acquisition and analysis.
Topics: Basic setup of Flow Cytometers; Principle of Cell Sorting; Parameters for Sorting; Alternative Separation Techniques (including magnetic bead separation (MACS)); Choice of Fluorochromes Depending on the type of Instrument and Application; Parameters of Analysis, Data Analysis and Interpretation; In-depth Discussion of Examples of Various Flow Cytometric Assays (e.g.; Cell Cycle Analysis, Cell Activation, Apoptosis, Immunology, Hematology, Biochemistry with Methodical Protocols); Multi-color Analysis (With Special Emphasis on How to Combine Fluorochromes and Fluorescence Compensation); Choosing the Right Controls for Data Analysis (Isotype Controls; Blocking with Antibodies).
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