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World Stem Cell Summit 2010
Sunday, June 1, 2008
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http://groups.yahoo.com/group/CNS_Healing
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[StemCells] 'Mood drugs' expand brains neural stem cell 'pool' (abstract)
Mood Stabilizing Drugs Expand the Neural Stem Cell Pool in the Adult
Brain Through Activation of Notch Signaling
First Published: May 8, 2008
* To whom correspondence should be addressed. E-mail:
shitoshi@nips.
Neural stem cells (NSCs) have attracted considerable attention as a
potential source of cells for therapeutic treatment of impaired areas
of the central nervous system. However, efficient and clinically
feasible strategies for expansion of the endogenous NSC pool are
currently unavailable. In this study, we demonstrate that mood
stabilizing drugs, which are used to treat patients with bipolar
disorder, enhance the self-renewal capability of mouse NSCs in vitro
and that this enhancement is achieved at therapeutically relevant
concentrations in the cerebrospinal fluid. The pharmacological
effects are mediated by the activation of Notch signaling in the NSC.
Treatment with mood stabilizers increased an active form of Notch
receptor and upregulated its target genes in neural stem/progenitor
cells, whereas co-culture with -secretase inhibitor or the presence
of mutation in the presenilin1 gene blocked the effects of mood
stabilizers. In addition, chronic administration of mood stabilizers
expanded the NSC pool in the adult brain, which subsequently
increased the cell supply to the olfactory bulb. We suggest that
treatment with mood stabilizing drugs could be used to facilitate
regeneration following insult to the central nervous system.
Mikito Higashi 1, Noriko Maruta 2, Alan Bernstein 3, Kazuhiro Ikenaka
1, Seiji Hitoshi 1*
1 Department of Physiological Sciences, School of Life Sciences,
Graduate University for Advanced Studies, Kanagawa 240-0193, Japan.;
Division of Neurobiology and Bioinformatics, National Institute for
Physiological Sciences, Okazaki 444-8787, Japan.
2 Department of Psychiatry, Health Service Center, University of
Tokyo, Tokyo 113-8655, Japan.
3 Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto,
Ontario M5G 1X5, Canada.
http://stemcells.
____________________________________________
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¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
StemCells subscribers may also be interested in these sites:
Children's Neurobiological Solutions
http://www.CNSfoundation.org/
Cord Blood Registry
http://www.CordBlood.com/at.cgi?a=150123
The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
____________________________________________
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¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
StemCells subscribers may also be interested in these sites:
Children's Neurobiological Solutions
http://www.CNSfoundation.org/
Cord Blood Registry
http://www.CordBlood.com/at.cgi?a=150123
The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
____________________________________________
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[StemCells] UK to make cloned SCs wo patient's consent
Soon, tissue of dead humans to be cloned
London (PTI): Scientists will soon be able to use tissue from dead
people to create cloned human stem cells for medical research, under
a legal change proposed by the British government.
Health Ministers have put forward the proposal that clinical
laboratories should be permitted to use stored human tissue to create
cloned embryonic stem cells even without the explicit consent of the
donor, 'The Sunday Times' reported. This would allow research to be
done on tissue donated for medical research as long as 30 years ago,
according to the Ministers in Britain.
Many laboratories have banks of stored tissues which act as DNA
libraries that can play a crucial role in finding cures for serious
disorders such as diabetes and motor neurone disease. Ministers have
until now insisted that scientists contact tissue donors to gain
explicit consent before DNA can be used to create cloned embryonic
stem cells.
However, leading scientists say gaining such consent is sometimes
impossible because the donors have died, donated anonymously or
cannot be contacted. They say the ban on using DNA without consent
could hold up vital research.
Now, the Ministers have tabled an amendment to the Human
Fertilisation and Embryology Bill, currently passing through
Parliament, which would allow stored tissue and cells to be used
without the explicit consent of donors. The amendment, which is
expected to be supported by most MPs, will be debated this week.
http://www.hinduonn
____________________________________________
«¤»¥«¤»§«¤»¥«¤»§«¤»¥«¤»«¤»¥«¤»§«¤»¥«¤»§«¤»¥«
¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
StemCells subscribers may also be interested in these sites:
Children's Neurobiological Solutions
http://www.CNSfoundation.org/
Cord Blood Registry
http://www.CordBlood.com/at.cgi?a=150123
The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
____________________________________________
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¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
«¤»¥«¤»§«¤»¥«¤»§«¤»¥«¤»«¤»¥«¤»§«¤»¥«¤»§«¤»¥«
¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
StemCells subscribers may also be interested in these sites:
Children's Neurobiological Solutions
http://www.CNSfoundation.org/
Cord Blood Registry
http://www.CordBlood.com/at.cgi?a=150123
The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
____________________________________________
«¤»¥«¤»§«¤»¥«¤»§«¤»¥«¤»«¤»¥«¤»§«¤»¥«¤»§«¤»¥«
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[StemCells] Mesenchymal reduce Acute Kidney Injury (abstract)
Human Bone Marrow-mesenchymal Stem Cells Accelerate Recovery of Acute
Renal Injury and Prolong Survival in Mice
First published online May 22, 2008
* To whom correspondence should be addressed. E-mail:
morigi@marionegri.
Abstract
Transplantation of bone marrow mesenchymal stem cells or stromal
cells (BM-MSC) from rodents has been identified as a strategy for
renal repair in experimental models of acute kidney injury (AKI), a
highly life-threatening clinical setting. The therapeutic potential
of BM-MSC of human origin has not been reported so far. Here, we
investigated whether human BM-MSC treatment could prevent AKI induced
by cisplatin and prolong survival in an immunodeficient mouse model.
Results showed that human BM-MSC infusion decreased proximal tubular
epithelial cell injury and ameliorated the deficit in renal function
resulting in reduced recipient mortality. Infused BM-MSC became
localized predominantly in peritubular areas and acted to reduce
renal cell apoptosis and to increase proliferation. BM-MSC also
induced protection against AKI-related peritubular capillary changes
consisting of endothelial cell abnormalities, leukocyte infiltration,
and low endothelial cell and lumen volume density as assessed by
morphometric analysis. These findings indicate that human MSC of bone
marrow origin hold potential to prolong survival in AKI and should be
considered for testing in a clinical trial.
____________
________
AUTHOR CONTRIBUTIONS: M.M.: Conception and design, collection and/or
assembly of data, data analysis and interpretation, manuscript
writing.; M.I.: Conception and design, provision of study material or
patients, data analysis and interpretation.
design, collection and/or assembly of data, data analysis and
interpretation, manuscript writing.; D.C.: Data analysis and
interpretation.
analysis and interpretation, manuscript writing.; C.R.: Collection
and/or assembly of data, data analysis and interpretation.
Data analysis and interpretation.
collection and/or assembly of data, data analysis and interpretation,
final approval of manuscript.; N.P.: Collection and/or assembly of
data.; C.Z.: Collection and/or assembly of data, data analysis and
interpretation.
A.R.: Data analysis and interpretation, collection and/or assembly of
data.; G.R.: Conception and design, administrative support, data
analysis and interpretation, manuscript writing, final approval of
manuscript.
Marina Morigi, Martino Introna, Barbara Imberti contributed equally
to this work.
Key Words. human mesenchymal stem cells, acute renal failure, tubular
cells, kidney repair
Marina Morigi 1*, Martino Introna 2, Barbara Imberti 1, Daniela Corna
1, Mauro Abbate 1, Cinzia Rota 1, Daniela Rottoli 1, Ariela Benigni
1, Norberto Perico 1, Carla Zoja 1, Alessandro Rambaldi 2, Andrea
Remuzzi 1, Giuseppe Remuzzi 3
1 `Mario Negri' Institute for Pharmacological Research, Via Gavazzeni
11, 24125 Bergamo, Italy
2 Laboratorio di Terapia Cellulare e Genica "G. Lanzani", Unit of
Hematology, Azienda Ospedaliera, Ospedali Riuniti di Bergamo via
Garibaldi 11/13, 24, 24124 Bergamo, Italy
3 `Mario Negri' Institute for Pharmacological Research, Via Gavazzeni
11, 24125 Bergamo, Italy; Unit of Nephrology and Dialysis, Azienda
Ospedaliera, Ospedali Riuniti di Bergamo, Largo Barozzi 1, 24128
Bergamo, Italy
http://stemcells.
____________________________________________
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¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
StemCells subscribers may also be interested in these sites:
Children's Neurobiological Solutions
http://www.CNSfoundation.org/
Cord Blood Registry
http://www.CordBlood.com/at.cgi?a=150123
The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
____________________________________________
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¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
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¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
StemCells subscribers may also be interested in these sites:
Children's Neurobiological Solutions
http://www.CNSfoundation.org/
Cord Blood Registry
http://www.CordBlood.com/at.cgi?a=150123
The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
____________________________________________
«¤»¥«¤»§«¤»¥«¤»§«¤»¥«¤»«¤»¥«¤»§«¤»¥«¤»§«¤»¥«
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[StemCells] Dental Pulp to Nerves (abstract)
First published online May 22, 2008
Adult Human Dental Pulp Stem Cells Differentiate Towards Functionally
Active Neurons Under Appropriate Environmental Cues
Abstract
Human adult dental pulp stem cells (DPSC) reside within the
perivascular niche of dental pulp and are thought to originate from
migrating cranial neural crest (CNC) cells. During embryonic
development, CNC cells differentiate into a wide variety of cell
types including neurons of the peripheral nervous system. Previously,
we have demonstrated that DPSC derived from adult human third molar
teeth differentiate into cell types reminiscent of CNC embryonic
ontology. We hypothesized that DPSC exposed to the appropriate
environmental cues would differentiate into functionally active
neurons. The data demonstrated that ex vivo expanded human adult DPSC
responded to neuronal inductive conditions both in vitro and in vivo.
Human adult DPSC, but not human foreskin fibroblasts (HFF) acquired a
neuronal morphology, and expressed neuronal specific markers at both
the gene and protein levels. Culture expanded DPSC also exhibited the
capacity to produce a sodium current consistent with functional
neuronal cells when exposed to neuronal inductive media. Furthermore,
the response of human DPSC and HFF to endogenous neuronal
environmental cues was determined in vivo using an avian xeno-
transplantation assay. DPSC expressed neuronal markers and acquired a
neuronal morphology following transplantation into the mesencephalon
of embryonic day two chicken embryo, while HFF maintained a thin
spindle fibroblastic morphology. We propose that adult human DPSC
provide a readily accessible source of exogenous stem/precursor cells
which have the potential for use in cell therapeutic paradigms to
treat neurological disease.
____________
Agnes Arthur 1, Grigori Rychkov 2, Songtao Shi 3, Simon Andrea Koblar
4, Stan Gronthos 5*
1 The Australian Research Council, Centre for the Molecular Genetics
of Development, University of Adelaide, Adelaide, 5005, SA,
Australia.; School of Molecular and Biomedical Science (Genetics),
University of Adelaide, Adelaide, 5005, SA, Australia.; Mesenchymal
Stem Cell Group, Division of Haematology, Institute of Medical and
Veterinary Science, Hanson Institute/Adelaide University, Adelaide,
5000, SA, Australia.
2 School of Molecular and Biomedical Science (Physiology)
of Adelaide, Adelaide, 5005, SA, Australia.
3 School of Dentistry, University of Southern California, Los
Angeles, 90089-0641, CA, USA.
4 The Australian Research Council, Centre for the Molecular Genetics
of Development, University of Adelaide, Adelaide, 5005, SA,
Australia.; School of Molecular and Biomedical Science (Genetics),
University of Adelaide, Adelaide, 5005, SA, Australia.
5 Mesenchymal Stem Cell Group, Division of Haematology, Institute of
Medical and Veterinary Science, Hanson Institute/Adelaide University,
Adelaide, 5000, SA, Australia.
* To whom correspondence should be addressed. E-mail:
stan.gronthos@
________
Author Contributions: A.A.: Conception and design, collection and/or
assembly of data, data analysis and interpretation, manuscript
writing.; G.R.: Collection and/or assembly of data, data analysis and
interpretation, manuscript writing.; S.S.: Conception and design,
provision of study material or patients.; S.K.: Conception and
design, data analysis and interpretation, manuscript writing,
financial support, final approval of manuscript.; S.G.: Conception
and design, data analysis and interpretation, manuscript writing,
financial support, final approval of manuscript.
S.A. Koblar and S. Gronthos contributed equally to this work.
Key Words. Dental Pup Stem Cells (DPSC), neuronal differentiation
http://stemcells.
____________________________________________
«¤»¥«¤»§«¤»¥«¤»§«¤»¥«¤»«¤»¥«¤»§«¤»¥«¤»§«¤»¥«
¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
StemCells subscribers may also be interested in these sites:
Children's Neurobiological Solutions
http://www.CNSfoundation.org/
Cord Blood Registry
http://www.CordBlood.com/at.cgi?a=150123
The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
____________________________________________
«¤»¥«¤»§«¤»¥«¤»§«¤»¥«¤»«¤»¥«¤»§«¤»¥«¤»§«¤»¥«
¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
«¤»¥«¤»§«¤»¥«¤»§«¤»¥«¤»«¤»¥«¤»§«¤»¥«¤»§«¤»¥«
¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
StemCells subscribers may also be interested in these sites:
Children's Neurobiological Solutions
http://www.CNSfoundation.org/
Cord Blood Registry
http://www.CordBlood.com/at.cgi?a=150123
The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
____________________________________________
«¤»¥«¤»§«¤»¥«¤»§«¤»¥«¤»«¤»¥«¤»§«¤»¥«¤»§«¤»¥«
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[StemCells] UK to make cloned hES without patient consent
Soon, tissue of dead humans to be cloned
London (PTI): Scientists will soon be able to use tissue from dead
people to create cloned human stem cells for medical research, under
a legal change proposed by the British government.
Health Ministers have put forward the proposal that clinical
laboratories should be permitted to use stored human tissue to create
cloned embryonic stem cells even without the explicit consent of the
donor, 'The Sunday Times' reported. This would allow research to be
done on tissue donated for medical research as long as 30 years ago,
according to the Ministers in Britain.
Many laboratories have banks of stored tissues which act as DNA
libraries that can play a crucial role in finding cures for serious
disorders such as diabetes and motor neurone disease. Ministers have
until now insisted that scientists contact tissue donors to gain
explicit consent before DNA can be used to create cloned embryonic
stem cells.
However, leading scientists say gaining such consent is sometimes
impossible because the donors have died, donated anonymously or
cannot be contacted. They say the ban on using DNA without consent
could hold up vital research.
Now, the Ministers have tabled an amendment to the Human
Fertilisation and Embryology Bill, currently passing through
Parliament, which would allow stored tissue and cells to be used
without the explicit consent of donors. The amendment, which is
expected to be supported by most MPs, will be debated this week.
http://www.hinduonn
____________________________________________
«¤»¥«¤»§«¤»¥«¤»§«¤»¥«¤»«¤»¥«¤»§«¤»¥«¤»§«¤»¥«
¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
StemCells subscribers may also be interested in these sites:
Children's Neurobiological Solutions
http://www.CNSfoundation.org/
Cord Blood Registry
http://www.CordBlood.com/at.cgi?a=150123
The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
____________________________________________
«¤»¥«¤»§«¤»¥«¤»§«¤»¥«¤»«¤»¥«¤»§«¤»¥«¤»§«¤»¥«
¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
«¤»¥«¤»§«¤»¥«¤»§«¤»¥«¤»«¤»¥«¤»§«¤»¥«¤»§«¤»¥«
¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯
StemCells subscribers may also be interested in these sites:
Children's Neurobiological Solutions
http://www.CNSfoundation.org/
Cord Blood Registry
http://www.CordBlood.com/at.cgi?a=150123
The CNS Healing Group
http://groups.yahoo.com/group/CNS_Healing
____________________________________________
«¤»¥«¤»§«¤»¥«¤»§«¤»¥«¤»«¤»¥«¤»§«¤»¥«¤»§«¤»¥«
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