Stem Cell: March 2008

Monday, March 31, 2008

Keyword News: [stem cell]

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Monday, March 31, 2008 11:31 PM PDT

Adult stem cell bill advances at the Capitol
9 News Denver Mon, 31 Mar 2008 4:42 PM PDT
DENVER - Colorado lawmakers took the first step on Monday toward advancing the non-controversial form of stem cell technology, much to Sheila Gannon's relief.

Researchers examine human embryonic stem cell genome
News-Medical-Net Mon, 31 Mar 2008 5:53 PM PDT
Stem cell researchers from UCLA used a high resolution technique to examine the genome, or total DNA content, of a pair of human embryonic stem cell lines and found that while both lines could form neurons, the lines had differences in the numbers of certain genes that could control such things as individual traits and disease susceptibility.

Stem cell research centre at Stanley Hospital
The Hindu Mon, 31 Mar 2008 12:37 PM PDT
CHENNAI: The first ever stem cell research centre in the government sector in Tamil Nadu will come up in Government Stanley Hospital on an outlay of Rs.15 crore. The Indian Council for Medical Research will fund the project. College Dean ...

Stem Cell Therapeutics Corp. Announces Year End 2007 Financial Results
CCNMatthews via Yahoo! Finance Mon, 31 Mar 2008 9:19 AM PDT
CALGARY, ALBERTA-- - Stem Cell Therapeutics Corp. today announced its financial results for the period ended December 31, 2007.

International Stem Cell Corporation Announces Manufacturing and Supply Agreement With ATCC
Business Wire via Yahoo! Finance Mon, 31 Mar 2008 6:30 AM PDT
OCEANSIDE, Calif.----International Stem Cell Corporation announced today that it has entered into an agreement with ATCC to manufacture living primary cells and cell culture products for worldwide distribution by ATCC into the biological research market.

Lecturer at ASU will speak on stem cell use for nervous system disorders
The San Angelo Standard-Times Mon, 31 Mar 2008 10:05 PM PDT
The use of stem cells in the treatment of nervous system disorders and dementia is the topic of a free public lecture at Angelo State University tonight - and the organizer says families should attend

CalbaTech Announces $999,926 Reduction in Debt; First Adult Stem Cell Harvesting Scheduled
PR Newswire via Yahoo! Finance Mon, 31 Mar 2008 11:07 AM PDT
CalbaTech, Inc. , the emerging life sciences company concentrating on the collection and banking of adult stem cells for possible future therapeutic uses, today announced the Company decreased debt by $999,926 in 2007.

BrainStorm Cell Therapeutics Opens New Onsite Animal House
Centre Daily Times Mon, 31 Mar 2008 10:56 AM PDT
BrainStorm Cell Therapeutics Inc. (OTCBB:BCLI), a leading developer of adult stem cell technologies and therapeutics, is pleased to announce the opening of a new animal house located at BrainStorm's research headquarters in Petach Tikvah, Israel.

Daniel Rubin: At late jazz great's request, a drive to recruit minority stem-cell donors
The Philadelphia Inquirer Mon, 31 Mar 2008 10:28 PM PDT
Saxophonist Michael Brecker was a reluctant poster boy for the bone-marrow disorder that killed him. Even when he could not find a match among the 10 million or so people on a registry of marrow donors, the tenor giant was uncomfortable letting his family use his name to recruit people who shared his genetic markers.

Stem cell research centre at Stanley
The Hindu Mon, 31 Mar 2008 2:51 PM PDT
ICMR will fund the project

See more news stories that match my keyword

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International Stem Cell Corporation...

Release #:1468-50460-rl-434148:

International Stem Cell Corporation Announces Manufacturing and Supply Agreement With ATCC

International Stem Cell Corporation (OTCBB:ISCO) announced today that it has entered into an agreement with ATCC (American Type Culture Collection, Manassas, Va.) to manufacture living primary cells and cell culture products for worldwide distribution by ATCC into the biological research market.

"We are proud of our relationship with ATCC. They are well known around the world as a leader in supplying biomaterials for critical research being conducted in the field of life sciences," according to Jeffrey Janus, President of ISCO. "Relationships with quality organizations such as ATCC are critical to ISCO's future success. This is yet another important step toward ISCO's becoming the industry leader in providing cells and cell products for therapy. We advance our therapeutic goals by generating revenue, scientific knowledge and infrastructure through research, product manufacture and sales."

International Stem Cell Corporation is the first company to perfect a method of creating human stem cells from unfertilized eggs. These cells, called "parthenogenetic" stem cells alleviate two critical problems inherent in cell transplantation today — immune rejection and the ethical issues associated with the use of fertilized human embryos — yet they retain the ability to differentiate into all tissue types.

"These products and the expertise of ISCO broaden the ATCC offering and complement our strong position in cell biology," said Michael Gove, ATCC VP for Marketing and Sales. "Offering primary cells and culture products will enhance our position as the world's leading biological resource center and provide our customers more high-quality reagents for their research."

ATCC, a global nonprofit, supports biological research by acquiring, authenticating, developing, preserving and distributing the world's most comprehensive source of cell and microbial reference cultures and reagents. ATCC functions as a biological repository, a research organization and a service provider for use by researchers in academic and industry laboratories.

ISCO, through its wholly owned subsidiary, Lifeline Cell Technology (Walkersville, MD) develops and manufactures optimized human cell culture products for research use. These products consist of frozen cells and cell culture media and reagents and include stem cell products.

About ATCC:

ATCC is the world's largest biological resource center and the most comprehensive source of reference cultures and reagents used by researchers in academic and industry laboratories. Since 1925, ATCC, also known as American Type Culture Collection, has set the standard for authentication and distribution of biological reference materials for life science research in the public and private sector. www.atcc.org.

About International Stem Cell Corporation (ISCO.OB):

International Stem Cell Corporation (ISCO) is a California biotechnology company focused on developing therapeutic and research products. ISCO's technology, Parthenogenesis, results in the creation of pluripotent human stem cell lines from unfertilized human eggs. ISCO scientists also have created the first Parthenogenetic homozygous stem cell line (phSC-Hhom-4) that can be a source of therapeutic cells that will not be immune rejected after transplantation into millions of individuals of differing sexes, ages and racial groups. These advancements offer the potential to create the first true "Stem Cell Bank" and address ethical issues by eliminating the need to use or destroy fertilized embryos. ISCO also produces and markets specialized cells and growth media worldwide for therapeutic research through its subsidiary Lifeline Cell Technology. For more information, visit the ISCO website at: www.internationalstemcell.com.

To subscribe to receive ongoing corporate communications please click on the following link: http://www.b2i.us/irpass.asp?BzID=1468&to=ea&s=0.

Forward-Looking Statements

Statements pertaining to future financial and/or operating results, future growth in research, technology, clinical development and potential opportunities for the company and its subsidiary, along with other statements about the future expectations, beliefs, goals, plans, or prospects expressed by management constitute forward-looking statements. Any statements that are not historical fact (including, but not limited to statements that contain words such as "will,""believes,""plans,""anticipates,""expects,""estimates,") should also be considered to be forward-looking statements. Forward-looking statements involve risks and uncertainties, including, without limitation, risks inherent in the development and/or commercialization of potential products, uncertainty in the results of clinical trials or regulatory approvals, need and ability to obtain future capital, and maintenance of intellectual property rights. Actual results may differ materially from the results anticipated in these forward-looking statements and as such should be evaluated together with the many uncertainties that affect the company's business, particularly those mentioned in the cautionary statements found in the company's Securities and Exchange Commission filings. The company disclaims any intent or obligation to update these forward-looking statements.

KEYWORDS: Stem Cells, Biotechnology, Parthenogenesis

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Kidney International Table of Contents alert Volume 73 Issue s108

KIDNEY INTERNATIONAL

April 2008 Volume 73 Number S108, pp S1 - S176

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----------------------
EDITORIAL
----------------------
Back to the future: It does not only happen in Hollywood
J C Divino Filho, C McIntyre, J V Povlsen and R Paniagua
http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Bra50E3

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RESEARCH ARTICLES
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Peritoneal dialysis: A clinical study of factors governing its
effectiveness
S T Boen
http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brbw0EB

Studies of peritoneal dialysis
J Gjessing
http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brby0ED

Epithelial-to-mesenchymal transition of mesothelial cells is an early
event during peritoneal dialysis and is associated with high
peritoneal transport
G del Peso, J A Jimenez-Heffernan, M A Bajo, L S Aroeira, A Aguilera,
A Fernandez-Perpen, A Cirugeda, M J Castro, R de Gracia, R
Sanchez-Villanueva, J A Sanchez-Tomero, M Lopez-Cabrera and R Selgas
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brbz0EE
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brb10Ez

Clinical outcomes and peritoneal histology in patients starting
peritoneal dialysis are related to diabetic status and serum albumin
levels
J-C Contreras-Velazquez, V Soto, Y Jaramillo-Rodriguez, L-I
Samaniego-Rios, V Quinones-Perez, M Avila, D Amato and R Paniagua
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brb20E1
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brb30E2

Benefits of preserving residual renal function in peritoneal dialysis
B Marron, C Remon, M Perez-Fontan, P Quiros and A Ortiz
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brb40E3
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brb50E4

Incremental peritoneal dialysis: Effects on the choice of dialysis
modality, residual renal function and adequacy
G Viglino, L Neri and S Barbieri
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brb60E5
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brb70E6

Contrasting clinical outcomes between different modes of peritoneal
dialysis regimens: Two center experiences in China
W Chen, Y Gu, Q-F Han and T Wang
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brb80E7
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcA0EI

Complementary use of peritoneal and hemodialysis: Therapeutic
synergies in the treatment of end-stage renal failure patients
H Kawanishi and C McIntyre
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcB0EJ
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcC0EK

Peritoneal dialysis for older people: Overcoming the barriers
E A Brown
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcD0EL
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcE0EM

Costs of home assistance for peritoneal dialysis: Results of a
European survey
M Dratwa
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcF0EN
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcG0EO

Improved patient//technique survival and peritonitis rates in patients
treated with automated peritoneal dialysis when compared to continuous
ambulatory peritoneal dialysis in a Mexican PD center
A Ramos Sanchez, C Madonia and R A Rascon-Pacheco
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcH0EP
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcI0EQ

Clinical outcome in children with acute renal failure treated with
peritoneal dialysis after surgery for congenital heart disease
K R Pedersen, V E Hjortdal, S Christensen, J Pedersen, K Hjortholm, S
H Larsen and J V Povlsen
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcJ0ER
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcK0ES

High volume peritoneal dialysis vs daily hemodialysis: A randomized,
controlled trial in patients with acute kidney injury
D P Gabriel, J T Caramori, L C Martim, P Barretti and A L Balbi
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcL0ET
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcM0EU

Nocturnal ultrafiltration profiles in patients on APD: Impact on fluid
and solute transport
N D Vega, R Gallego, E Oliva, F Henriquez, L Palop, J C Divino Filho
and B Lindholm
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcN0EV
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcO0EW

The contribution of combined crystalloid and colloid osmosis to fluid
and sodium management in peritoneal dialysis
P Freida, M Wilkie, S Jenkins, F Dallas and B Issad
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcP0EX
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcQ0EY

The leptin//adiponectin ratio: Potential implications for peritoneal
dialysis
D Teta, M Maillard, G Halabi and M Burnier
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcR0EZ
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcS0Ea

Effects of peritoneal dialysis fluid biocompatibility on baroreflex
sensitivity
S G John, N M Selby and C W McIntyre
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcT0Eb
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcU0Ec

Echocardiographic, electrocardiographic and blood pressure changes
induced by icodextrin solution in diabetic patients on peritoneal
dialysis
R Paniagua, O Orihuela, M-d-J Ventura, M avila-Diaz, A Cisneros, M
Vicente-Martinez, M-d-C Furlong, Z Garcia-Gonzalez, D Villanueva,
M-d-C Prado-Uribe, G Alcantara and D Amato
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcV0Ed
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcW0Ee

Amino-acid-based peritoneal dialysis solution improves amino-acid
transport into skeletal muscle
M Asola, K Virtanen, K Nagren, S Helin, M Taittonen, H Kastarinen, B
Anderstam, J Knuuti, K Metsarinne and P Nuutila
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcX0Ef
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcY0Eg

Use of new peritoneal dialysis solutions in children
A Canepa, E Verrina and F Perfumo
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0BrcZ0Eh
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brca0Eo

The Brazilian Peritoneal Dialysis Multicenter Study (BRAZPD):
Characterization of the cohort
N Fernandes, M G Bastos, H V Cassi, N L Machado, J A Ribeiro, G
Martins, O Mourao, K Bastos, S R Ferreira Filho, V M Lemos, M Abdo, M
T I Vannuchi, A Mocelin, S L Bettoni, R V Valenzuela, M M Lima, S W
Pinto, M C Riella, A R Qureshi, J C Divino Filho and R Pecoits-Filho
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brcb0Ep
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brcc0Eq

Phosphorus control in peritoneal dialysis patients
A Yavuz, F F Ersoy, P S Passadakis, P Tam, D M Evaggelos, K P
Katopodis, C Ozener, F Akcicek, T Camsari, K Ates, R Ataman, G J
Vlachojannis, N A Dombros, C Utas, T Akpolat, S Bozfakioglu, G Wu, I
Karayaylali, T Arinsoy, C P Stathakis, M Yavuz, D J Tsakiris, A C
Dimitriades, M E Yilmaz, M Gultekin, G Suleymanlar and D G Oreopoulos
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brcd0Er
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brce0Es

Rio de La Plata study: A multicenter, cross-sectional study on
cardiovascular risk factors and heart failure prevalence in peritoneal
dialysis patients in Argentina and Uruguay
G Moretta, A J Locatelli, L Gadola, J De Arteaga, L Sola, N Caporale,
J Schargorodsky, E Ducasse, G Pastorino, N Marchetta, W Espeche and Z
Ortiz
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brcf0Et
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brcg0Eu

Dialysis outcomes in Colombia (DOC) study: A comparison of patient
survival on peritoneal dialysis vs hemodialysis in Colombia
M Sanabria, J Munoz, C Trillos, G Hernandez, C Latorre, C S Diaz, S
Murad, K Rodriguez, A Rivera, A Amador, F Ardila, A Caicedo, D
Camargo, A Diaz, J Gonzalez, H Leguizamon, P Lopera, L Marin, I Nieto
and E Vargas
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brch0Ev
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brci0Ew

Automated peritoneal dialysis as a lifesaving therapy in an emergency
room: Report of four cases
M B Ilabaca-Avendano, G Yarza-Solorzano, J Rodriguez-Valenzuela, G
Arcinas-Fausto, V Ramirez-Hernandez, D A Hernandez-Hernandez and L A
Jauregui-Flores
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brcj0Ex
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ej1R0Xztnp0Hiy0Brck0Ey

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International Stem Cell Corporation...

Release #:1468-50456-rl-434148:

International Stem Cell Corporation Announces Manufacturing and Supply Agreement With ATCC

OCEANSIDE, Calif.--(International Stem Cell Corporation (OTCBB:ISCO) announced today that it has entered into an agreement with ATCC (American Type Culture Collection, Manassas, Va.) to manufacture living primary cells and cell culture products for worldwide distribution by ATCC into the biological research market.

About ATCC:

About International Stem Cell Corporation (ISCO.OB):

To subscribe to receive ongoing corporate communications please click on the following link: http://www.b2i.us/irpass.asp?BzID=1468&to=ea&s=0.

Forward-Looking Statements

Statements pertaining to future financial and/or operating results, future growth in research, technology, clinical development and potential opportunities for the company and its subsidiary, along with other statements about the future expectations, beliefs, goals, plans, or prospects expressed by management constitute forward-looking statements. Any statements that are not historical fact (including, but not limited to statements that contain words such as "will," "believes," "plans," "anticipates," "expects," "estimates,") should also be considered to be forward-looking statements. Forward-looking statements involve risks and uncertainties, including, without limitation, risks inherent in the development and/or commercialization of potential products, uncertainty in the results of clinical trials or regulatory approvals, need and ability to obtain future capital, and maintenance of intellectual property rights. Actual results may differ materially from the results anticipated in these forward-looking statements and as such should be evaluated together with the many uncertainties that affect the company's business, particularly those mentioned in the cautionary statements found in the company's Securities and Exchange Commission filings. The company disclaims any intent or obligation to update these forward-looking statements.

KEYWORDS: Stem Cells, Biotechnology, Parthenogenesis

Contact:

International Stem Cell Corporation Kenneth C. Aldrich, 760-940-6383 Chairman, CEO kaldrich@intlstemcell.com William B. Adams, 760-940-6383 Chief Financial Officer wadams@intlstemcell.com

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Nature Methods Contents: April 2008 Volume 5 pp 275 - 369

NATURE METHODS

April 2008 Volume 5 Number 4, pp 275 - 369

Visit Nature Methods online to browse the journal.

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----------------------
EDITORIAL
----------------------
Compare and conquer p275
Experimental comparisons of methods, technology platforms or reagents
are time-consuming and expensive, but hugely beneficial. Enter the
Analysis article format.
doi:10.1038/nmeth0408-275
http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSC0Ex

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CORRESPONDENCE
----------------------
Cyclic nucleotide analogs as probes of signaling
pathways pp277 - 278
Heiko Poppe et al.
doi:10.1038/nmeth0408-277
http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSD0Ey

Whole genome-amplified DNA: insights and imputation pp279 - 280
Yik Y Teo et al.
doi:10.1038/nmeth0408-279
http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSE0Ez

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RESEARCH HIGHLIGHTS
----------------------
Protein suicide highlights the cell cycle p283
Daniel Evanko
doi:10.1038/nmeth0408-283
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSF0E1
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSG0E2

Mapping the plant methylome pp284 - 285
Nicole Rusk
doi:10.1038/nmeth0408-284a
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSH0E3
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSI0E4

Insulating gene expression pp284 - 285
Katherine Stevens
doi:10.1038/nmeth0408-284b
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSJ0E5
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSK0E6

News in brief p285
doi:10.1038/nmeth0408-285
http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSL0E7

DNA synthesis lights up p286
Allison Doerr
doi:10.1038/nmeth0408-286
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSM0E8
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSN0EA

ChIP-chip put to the test p288
Veronique Kiermer
doi:10.1038/nmeth0408-288
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSO0EB
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSP0EC

----------------------
COMMENTARY
----------------------
Disaccharide structure code for the easy representation of
constituent oligosaccharides from glycosaminoglycans pp291 - 292
Here we introduce a new shorthand nomenclature for designating the
disaccharide subunit structure of all glycosaminoglycans as a way
to compare compositions and describe linear sequences. Each
disaccharide structural code (DSC) comprises four alphanumeric
characters that reflect the actual substituents and isomeric
characteristics of each component sugar. Larger structures can be
represented easily by forming concatamers. The structural
transparency of this new nomenclature eliminates the need for
ambiguous acronyms or difficult numeric representations.
Roger Lawrence et al.
doi:10.1038/nmeth0408-291
http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSQ0ED

----------------------
NEWS AND VIEWS
----------------------
Expanding the toolbox for remote control of neuronal
circuits pp293 - 295
Advances in methods that allow targeted remote control of neuronal
activity open new possibilities for investigating and manipulating
the function of neuronal circuits in vivo.
Thomas Knopfel
doi:10.1038/nmeth0408-293
http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSR0EE

SNPing and chipping away at the fly genome pp295 - 296
Although Drosophila melanogaster offers a variety of refined genetic
techniques, it has lagged behind other model organisms in the
high-resolution genotyping arena. A newly developed set of tools
addresses this deficiency and provides a very welcome addition to
the fly geneticists' armory.
Steven Russell
doi:10.1038/nmeth0408-295
http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSS0EF

Levers and fulcrums: progress in cis-regulatory motif models pp297 - 298
A new set of computational methods provide association of
transcription factor binding motifs to gene function.
Ewan Birney
doi:10.1038/nmeth0408-297
http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrST0EG

----------------------
BRIEF COMMUNICATIONS
----------------------
Genetic control of neuronal activity in mice conditionally expressing
TRPV1 pp299 - 302
Benjamin R Arenkiel et al.
doi:10.1038/nmeth.1190
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSU0EH
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSV0EI

Activation of protein splicing with light in yeast pp303 - 305
Amy B Tyszkiewicz and Tom W Muir
doi:10.1038/nmeth.1189
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSW0EJ
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSX0EK

Genome-wide mapping of allele-specific protein-DNA interactions
in human cells pp307 - 309
Nathaniel D Maynard et al.
doi:10.1038/nmeth.1194
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSY0EL
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSZ0EM

In vivo immunogold labeling confirms large-scale chromatin folding
motifs pp311 - 313
Igor Kireev et al.
doi:10.1038/nmeth.1196
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSa0ET
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSb0EU

Identification of cross-linked peptides from large sequence
databases pp315 - 318
Oliver Rinner et al.
doi:10.1038/nmeth.1192
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSc0EV
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSd0EW

A quantitative analysis software tool for mass spectrometry-based
proteomics pp319 - 322
Sung Kyu Park, John D Venable, Tao Xu and John R Yates, III
doi:10.1038/nmeth.1195
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSe0EX
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSf0EY

----------------------
ARTICLES
----------------------
High-resolution, high-throughput SNP mapping in Drosophila
melanogaster pp323 - 329
Doris Chen et al.
doi:10.1038/nmeth.1191
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSg0EZ
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSh0Ea

Photochemical control of endogenous ion channels and cellular
excitability pp331 - 338
Doris L Fortin et al.
doi:10.1038/nmeth.1187
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSi0Eb
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSj0Ec

Direct measurement of protein dynamics inside cells using a
rationally designed photoconvertible protein pp339 - 345
Tomoki Matsuda, Atsushi Miyawaki and Takeharu Nagai
doi:10.1038/nmeth.1193
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSk0Ed
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSl0Ee

Systematic identification of mammalian regulatory motifs' target
genes and functions pp347 - 353
Jason B Warner et al.
doi:10.1038/nmeth.1188
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSm0Ef
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSn0Eg

----------------------
PROTOCOL
----------------------
A sensitive direct human telomerase activity assay pp355 - 360
Scott B Cohen and Roger R Reddel
doi:10.1038/nmeth.f.209
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSo0Eh
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSp0Ei

----------------------
TECHNOLOGY FEATURE
----------------------
RNAi technologies: a screen whose time has arrived pp361 - 368
Researchers are finally reaping the rewards of large-scale RNA
interference (RNAi) screens in mammalian cells, obtaining insights
into a wide range of biological questions. Nathan Blow takes a look
at the technology advances spearheading this revolution.
Nathan Blow
doi:10.1038/nmeth0408-361
http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSq0Ej

----------------------
ADDENDUM
----------------------
Addendum: Protein production and purification p369.
Structural Genomics Consortium, Architecture et Fonction des
Macromolecules Biologiques, Berkeley Structural Genomics Center,
China Structural Genomics Consortium, Integrated Center for
Structure and Function Innovation, Israel Structural Proteomics
Center, Joint Center for Structural Genomics, Midwest Center for
Structural Genomics, New York Structural GenomiX Research Center
for Structural Genomics, Northeast Structural Genomics Consortium,
Oxford Protein Production Facility, Protein Sample Production
Facility, Max Delbruck Center for Molecular Medicine, RIKEN
Structural Genomics/Proteomics Initiative & SPINE2-Complexes
doi:10.1038/nmeth0408-369
http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSr0Ek

----------------------
APPLICATION NOTES
----------------------
Refining epifluorescence imaging and analysis with automated
multiple-band flat-field correction
Douglas Wood, Gilbert Feke, Douglas Vizard and Rao Papineni
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSs0El
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSt0Em

Selecting a cyclic AMP kit for assaying GPCR target activation
Jean-Luc Tardieu
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSu0En
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyF0Xztnp0Hi80BrSv0Eo

=====================================================================
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NATURE CLINICAL PRACTICE ONCOLOGY

April 2008 Volume 5 Number 4

Visit Nature Clinical Practice Oncology online to browse the journal.

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----------------------
EDITORIAL
----------------------
Strong inference
Vincent T DeVita Jr
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPO0EE

----------------------
VIEWPOINT
----------------------
Artificial ultraviolet sources and skin cancers: rationale for restricting
access to sunbed use before 18 years of age
Philippe Autier and Peter Boyle
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPP0EF

----------------------
RESEARCH HIGHLIGHTS
----------------------
TP53 mutation is associated with poor survival in patients with head
and neck cancer
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPQ0EG

Stroma-specific TP53 alterations correlate with clinical outcome in
breast cancer
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPR0EH

Capecitabine and oxaliplatin are effective treatments in advanced
esophagogastric cancer
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPS0EI

Chemoradiotherapy: standard treatment for Hodgkin's disease with favorable
prognosis
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPT0EJ

Prognostic value of pretreatment quality of life in patients with head
and neck cancer
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPU0EK

Adjuvant chemotherapy improves outcome in women with estrogen-receptor-poor
breast cancer
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPV0EL

HER2 status affects responsiveness to adjuvant anthracyclines
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPW0EM

Letrozole is superior to tamoxifen for endocrine-responsive breast cancer
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPX0EN

Lymphocyte-predominant Hodgkin's lymphoma: patient characteristics and
outcomes
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPY0EO

----------------------
PRACTICE POINTS
----------------------
Is total thyroidectomy the procedure of choice for papillary thyroid
cancer?
Robert Udelsman
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPZ0EP

Bevacizumab in combination with irinotecan for patients with recurrent
glioblastoma multiforme
David Schiff and Benjamin Purow
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPa0EW

What is the relevance of determining EGFR-variant-III status in
glioblastomas?
Antonio MP Omuro
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPb0EX

A comparison of chemotherapy plus surgery with surgery alone for localized
esophageal cancer
Dirk Rades and Steven E Schild
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPc0EY

Is trastuzumab associated with adverse cardiac effects in patients with
breast cancer?
Michael S Ewer and Daniel J Lenihan
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPd0EZ

----------------------
REVIEWS
----------------------
Angiogenesis as a strategic target for ovarian cancer therapy
Whitney A Spannuth, Anil K Sood and Robert L Coleman
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPe0Ea

Metastasis: a therapeutic target for cancer
Patricia S Steeg and Dan Theodorescu
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPf0Eb

Technology Insight: water diffusion MRI-a potential new biomarker
of response to cancer therapy
Daniel M Patterson, Anwar R Padhani and David J Collins
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPg0Ec

----------------------
CASE STUDY
----------------------
Methotrexate-associated mantle-cell lymphoma in an elderly man with
myasthenia gravis
Huy Tran, Catherine Cheung, Devinder Gill, Ujjwal Dua, Jamie Nourse,
Richard Boyle and Maher K Gandhi
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPh0Ed

----------------------
ARTICLE RESPONSE
----------------------
Sentinel lymph-node false positivity in melanoma
Alexander CJ van Akkooi, Johannes HW de Wilt, Christiane Voit,
Cees Verhoef, Stefan Suciu and Alexander MM Eggermont
http://ealerts.nature.com/cgi-bin24/DM/y/ejxs0Xztnp0HjM0BrPi0Ee

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Kidney International Table of Contents alert Volume 73 Issue 8

KIDNEY INTERNATIONAL

April 2008 Volume 73 Number 8, pp 899 - 980

----------------------

NEW from KI: the Kidney Podcast! Visit www.nature.com/ki/podcast today!
To listen as top researchers discuss their work with KI Editors, visit www.nature.com/ki/podcast/index.html#Episodes.

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Recommend now at http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BjIp0ES

----------------------
IN THIS ISSUE
----------------------
In this Issue
http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrQ60Ek

----------------------
NEPHROLOGISTS SANS FRONTI[EGRAVE]RES
----------------------
Nephrologists Sans Frontieres: How I became a teacher
Burton D Rose
http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrQ70El

----------------------
COMMENTARIES
----------------------
Connecting the interpodocyte slit diaphragm and actin dynamics: Emerging role for the nephrin signaling complex
S Lehtonen
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrQ80Em
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRA0Ew

The treatment of acute interstitial nephritis: More data at last
G B Appel
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRB0Ex
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRC0Ey

Cardiac arrests in hemodialysis patients: an ongoing challenge
M Ostermann
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRD0Ez
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRE0E1

Supersized kidneys: Lessons from the preclinical obese kidney
V D D'Agati and G S Markowitz
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRF0E2
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRG0E3

----------------------
JOURNAL CLUB
----------------------
Journal Club
http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRH0E4

----------------------
MINIREVIEW
----------------------
Stem cells in the embryonic kidney
R Nishinakamura
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRI0E5
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRJ0E6

Aminoacidurias: Clinical and molecular aspects
S M R Camargo, D Bockenhauer and R Kleta
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRK0E7
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRL0E8

----------------------
ORIGINAL ARTICLES
----------------------
Decreased tyrosine phosphorylation of nephrin in rat and human nephrosis
K Uchida, K Suzuki, M Iwamoto, H Kawachi, M Ohno, S Horita and K Nitta
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRM0EA
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRN0EB

Outcome of cardiac arrests attended by emergency medical services staff at community outpatient dialysis centers
T R Davis, B A Young, M S Eisenberg, T D Rea, M K Copass and L A Cobb
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRO0EC
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRP0ED

Early steroid treatment improves the recovery of renal function in patients with drug-induced acute interstitial nephritis
E Gonzalez, E Gutierrez, C Galeano, C Chevia, P de Sequera, C Bernis, E G Parra, R Delgado, M Sanz, M Ortiz, M Goicoechea, C Quereda, T Olea, H Bouarich, Y Hernandez, B Segovia and M Praga
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRQ0EE
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRR0EF

Renal injury in the extremely obese patients with normal renal function
A Serra, R Romero, D Lopez, M Navarro, A Esteve, N Perez, A Alastrue and A Ariza
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRS0EG
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRT0EH

Prevalence and severity of disordered mineral metabolism in Blacks with chronic kidney disease
O M Gutierrez, T Isakova, D L Andress, A Levin and M Wolf
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRU0EI
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRV0EJ

Lower blood pressure and risk of recurrent stroke in patients with chronic kidney disease: PROGRESS trial
T Ninomiya, V Perkovic, M Gallagher, M Jardine, A Cass, H Arima, C Anderson, B Neal, M Woodward, T Omae, S MacMahon and J Chalmers
Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRW0EK
Article: http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRX0EL

----------------------
THE RENAL CONSULT
----------------------
Uromodulin storage disease
S H Nasr, J P Lucia, S J Galgano, G S Markowitz and V D D'Agati
http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRY0EM

----------------------
MAKE YOUR DIAGNOSIS
----------------------
The Case [mid] Familial renal failure with ocular abnormality
J Bacchetta, C Habault, A Liutkus and P Cochat
http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRZ0EN

----------------------
NEPHROLOGY IMAGE
----------------------
Acute allergic interstitial nephritis and eosinophiluria
M Kaye and R F Gagnon
http://ealerts.nature.com/cgi-bin24/DM/y/ejyC0Xztnp0Hiy0BrRa0EU

----------------------

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Sunday, March 30, 2008

Keyword News: [stem cell]

Yahoo! Alerts

Yahoo! News - My Alerts - Edit Alert

Sunday, March 30, 2008 11:31 PM PDT

Stem cell research could appear on November ballot
The State News Sun, 30 Mar 2008 6:54 PM PDT
The fate of embryonic stem cell research in Michigan might rest in voters' hands if a grassroots campaign collects enough signatures by July 7. The Stem Cell Research Ballot Question Committee needs 380,126 signatures by its July deadline to put embryonic stem cell research on the November ballot.

Scripps, Torrey leaders avoid stem-cell subject
The Palm Beach Post Sun, 30 Mar 2008 9:16 PM PDT
Stem-cell research is a hard topic to dance around when you're part of a panel discussion on biotechnology.

New online stem cell patent database
News-Medical-Net Sun, 30 Mar 2008 4:23 PM PDT
StemCellPatents.com, an organization dedicated to the advancement of stem cell commercialization, has announced the launch of its newly re-designed free database covering US issued patents dealing with stem cells.

Cardinal agrees stem cell meeting
Channel 4 Sun, 30 Mar 2008 2:07 AM PDT
The leader of Scotland's Roman Catholic Church who attacked the Government's controversial embryology laws has agreed to meet scientists involved in stem cell research.

See more news stories that match my keyword

4 Stem Cell Patents Issued Last Week

View this message online

Dear manoj kumar valluru,

Last week 4 patents of relevance to the area of stem cells were issued.

# 7,347,876 (Patent Spotlight), describes how to expand limbal stem cells using amniotic membranes as a support matrix.
# 7,348,015 cancer vaccine by inserting bacterial immune evasion gene into the mammalian cancer cell.
# 7,347,999 teaches how to reconstitute a hematopoietic system.
# 7,349,740 an example of how to write strong claims.

Method for expansion of epithelial stem cells

Patent Number: 7,347,876

Assignee | Inventor

Corneal transplants are performed as a result of chemical or inflammatory injury to the clear part of the eye. Efficacy of corneal transplantation is decreased when the limbal stem cells which produce corneal epithelium are damaged or defective. Accordingly, transplantation of limbal stem cells has been performed clinically. A report in 1999 documented positive effects in some of the 70 patients treated. One of the issues with limbal stem cell transplantation is lack of donors or poor quality of the donor graft. The current patent addresses this problem by expanding ex vivo corneal epithelial stem cell.

The current patent has 2 independent claims. The first one covers "a surgical graft" that is made of:

- an amniotic membrane

- an extracellular matrix

- epithelial stem cells.

The second independent claim covers "a method for creating a surgical graft" through the steps of

- placing healthy epithelial stem cells in an isolated amniotic membrane

- enabling healthy epithelial stem cells to expand on the membrane

A very interesting point is that while the filing date of the patent is 2001, a recent publication (Yeh et al. Cryopreservation of Human Limbal Stem Cells Ex Vivo Expanded on Amniotic Membrane. Cornea 2008 Apr;27(3):327-333) not only demonstrated that limbal stem cells can be expanded in vitro on amniotic membranes but also that they can be cryopreserved for later use.

Noteworthy for people interested in this patent, an issued patent exists covering methods of collecting limbal stem cells, as well as the expansion of conjunctival stem cells is also patented.

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Mesenchymal Stem Cells in the Eye

Sunday March 30th, 2008 @ 20:24:11 EST

Hyderabad, India -

Mesenchymal stem cells are one of the most clinically advanced forms of stem cell therapy after hematopoietic stem cell transplantation. The conventional source for mesenchymal stem cells has been bone marrow, however these cells can also be extracted from a variety of other tissues such as teeth, placenta, cord blood, and menstrual blood.

A recent publication (Polisetty et al. Mesenchymal cells from limbal stroma of human eye. Mol Vis. 2008 Mar 4;14:431-42) describes mesenchymal stem cells found in the limbus of the human eye.

The investigators collected limbal tissues from patient biopsy samples during eye surgery. Cells with a spindle-like morphology were expanded as adherent cells in a medium containing EGF, insulin, and fetal calf serum.

The "mesenchymal" cells expanded from the limbus possessed a similar phenotype to bone marrow derived mesenchymal stem cells. For example, they expressed CD90, CD105, and CD29 and lacked expression of CD34, and CD45. Additionally, the limbal derived mesenchymal cells were capable of differentiating into bone, fat and cartilage tissue.

The identification of various subsets of stem cells in the human limbus is important since clinically limbal stem cell transplants have been performed with some degree of success. For example, a paper from 1999 (Tsubota et al. Treatment of severe ocular-surface disorders with corneal epithelial stem-cell transplantation. N Engl J Med 1999 Jun 3;340(22):1697-703) describes 70 corneal epithelial stem cell transplants, in which a statistically significant improvement in vision was observed.

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